FIGURE 3.

Repeated, acute Cch treatment maximizes Arc protein expression. Representative Western blot and quantitative analysis show Arc protein expression in cells treated repeatedly (up to 6 times) with Cch followed by washout. Each Cch application lasted for 2-min, and 10-min intervals separated the onset of consecutive applications. All lysates were prepared 120 min after beginning the initial Cch application. Arc levels are normalized to GAPDH and expressed in percent of Arc expression induced by chronic Cch treatment. Asterisks indicate a significant increase relative to single, acute Cch stimulation (p < 0.05; n = 4).