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. 2012 May 14;287(26):22354–22366. doi: 10.1074/jbc.M112.376491

FIGURE 5.

FIGURE 5.

Thapsigargin triggers rapid proteasomal degradation of Cch-induced Arc protein. A, representative blot and quantitative analysis show Arc protein expression in cells stimulated with Cch and treated simultaneously with MG-132 (MG) or vehicle (veh). Cells additionally received thapsigargin (thapsi) or vehicle 30 min after the start of Cch stimulation. B, representative blot and quantitative analysis show Arc protein expression in cells stimulated with Cch and treated simultaneously with MG-132 or vehicle. Cells additionally received BAPTA-AM (bapta) or vehicle 30 min after the start of Cch stimulation. Arc levels are normalized to GAPDH and expressed in percent of vehicle treatment. Asterisks indicate a significant change relative to vehicle or between groups (p < 0.05; n = 4). C, representative blot shows Arc protein expression in cells stimulated with Cch, then treated with BAPTA-AM and thapsigargin, respectively. Additional controls are provided in Fig. S5.