FIG. 3.

CSPG expression at the interface with dissociated DRG cells. (A) Astrocyte CSPG immunoreactivity was assessed using immunostaining and confocal microscopy, for CS56 (red) and Hoechst (blue) at days 5, 10, and 15 in culture. Representative confocal projections are shown of astrocytes at the interface with dissociated DRG cells, and away from the interface, in an astrocyte-only region at high magnification. Scale bar=100 μm. (B) Quantification of the volume of CSPG staining revealed a trend toward greater CSPG staining at the interface with DRG cells although these differences were not significantly different at any time point. CSPG, chondroitin sulfate proteoglycan. Color images available online at www.liebertonline.com/tec