Skip to main content
. 2011 Nov 28;590(Pt 4):763–776. doi: 10.1113/jphysiol.2011.219907

Figure 7. Application of hypertonic sucrose reveals a smaller readily releasable pool in Ophn-1−/y neurons.

Figure 7

A, representative traces of responses to sucrose solution from Ophn-1+/y (upper panel) and Ophn-1-/y neurons (lower panel). Under normal conditions (left panel) spontaneous IPSCs were observed, but at a lower frequency in Ophn-1-/y neurons. Application of sucrose (5 s) to the dendrites of granule cells increased the frequency of spontaneous IPSCs and increased holding current (centre panel), which reversed on wash (right panel). B, RRP size was estimated from the charge transfer in response to sucrose divided by the mean charge of miniature IPSCs recorded in the presence of 1 μm TTX. RRP is smaller in Ophn-1-/y neurons (open bars). *P < 0.05.