Figure 3.

A second closely timed retrieval results in rapid dephosphorylation of synaptic GluR1(Ser845). (A) Procedure for the conditions that varied the number of retrievals and time until sacrifice following the final retrieval event. Crude synaptosomal membrane fractions were then collected. (B) Synaptic GluR1(Ser845) phosphorylation is significantly increased at 60 min (n = 10), but not immediately (n = 9) after retrieval relative to no retrieval controls (n = 7). The presentation of a second retrieval 1 h after the first (n = 10) results in a rapid dephosphorylation of synaptic GluR1(Ser845). (C) There were no significant differences in total synaptic GluR1 expression. (*) P < 0.05 from no retrieval controls.