Fig. 4.
Experimental design for mechanical and biological characterization of gradient samples. Each gradient sample (6 cm × 1 cm × 3 mm) is cut in to six segments (1 cm × 1 cm × 3 mm). To assay the cell state in each gel segment, the segments are cut into five equal sections (1 cm × 2 mm × 3 mm) for use in the five assays indicated.