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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Cancer Cell. 2012 May 25;21(5):680–693. doi: 10.1016/j.ccr.2012.03.043

Figure 3. ERMS-propagating cells express myf5-GFP but not the mylz2-mCherry differentiated muscle marker.

Figure 3

(A) Schematic of experimental design.

(B-D) A primary ERMS arising in syngeneic myf5-GFP/mylz2-mCherry transgenic zebrafish (35 dpf). Broken black line denotes tumor area.

(E-G) Fluorescent-labeled ERMS engraft into syngeneic secondary recipient animals when transplanted with unsorted primary ERMS cells.

(H-K) FACS plots of fluorescent-labeled ERMS cells isolated from secondary recipient fish following two rounds of FACS.

(L-R) Transplantation of myf5-GFP+/mylz2-mCherry-negative FACs sorted cells induced ERMS in tertiary transplant animals and (S-U) quaternary recipients. Hematoxylin and eosin stained sections (C,F,M,T) and FACS (D,G,N,U) of primary and serially passaged ERMS. Scale bars equal 2 mm (B, E, L and S) and 100 μm (C, F, M and T).