Figure 3.

Memory 2C cells from spleen of B6 mice do not preferentially traffic to the prostate of TRP-SIY mice. Memory 2C (Thy1.1⁺CD8⁺) cells were purified from spleen of WSN-SIY-infected B6 (Thy1.2) mice (30 dpi) and ∼5×10⁵ cells were transferred into TRAMP and TRP-SIY (Thy1.2) recipient mice. One and half days later, transferred 2C cells (Thy1.1⁺CD8⁺) were quantified by cell counting and flow cytometry in the prostate, spleen and PDLN. The total 2C cell numbers are shown in each organ. Each symbol represents one mouse (n=4–6 per group, pooled from two separate experiments). Solid lines indicate the averages. B6, C57BL/6; dpi, days post-infection; PDLN, prostate draining lymph nodes; SIY, SIYRYYGL; TRAMP, TRansgenic Adenocarcinoma of the Mouse Prostate; TRP-SIY, TRAMP mice expressing SIY antigen in prostate tumor tissue; WSN-SIY, influenza A/WSN virus expressing the SIY epitope.