Table 2. Geomyces destructans and G. pannorum target gene fragments used for multiple gene genealogic analyses, New York, USA.
| Gene* | Homology (GenBank accession no.) | Amplicon size/ sequence used for comparison, bp | Primer sequence, 5′ → 3′† | G. destructans/G. pannorum GenBank accession nos. |
|---|---|---|---|---|
|
ALR
|
Penicillium
marneffei (XP_002152078.1) |
654/534 |
V1905 (f): CGGAGTGAGATTTATGACGGC |
HQ834314–HQ834329/HQ834330 |
| V1904 (r):
CGTCCATCCCAGACGTTCATC | ||||
|
Bpntase
|
Glomerella
graminicola (EFQ33509.1) |
921/745 |
V1869 (f): TCAGACGGACTCGGAGGGCAAG |
HQ834331–HQ834346/HQ834347 |
| V1926 (r):
TCGGTTACAGAGCCTCAGTCG | ||||
|
DHC1
|
Sordaria
macrospora (CBI53717.1) |
597/418 |
V1906 (f): GGATGATTCGGTCACCAAACAG |
HQ834348–HQ834363/HQ834364 |
| V1907 (r):
ACAGCAAACACAGCGCTGCAAG | ||||
|
GPHN
|
Ajellomyces
capsulatus (EEH06836.1) |
659/525 |
V1918 (f): CACTATTACATCGCCAGGCTC |
HQ834365–HQ834380/HQ834381 |
| V1919 (r):
CTAAACGCAGGCACTGCCTC | ||||
|
PCS
|
A.
capsulatus
(EEH08767.1) |
920/749 |
V1929 (f): AGGCTGCGATTGCTGAGTGC |
HQ834382–HQ834397/HQ834398 |
| V1873 (r):
CCTTATCCAGCTTTCCTTGGTC | ||||
|
POB3
|
Pyrenophora
tritici-repentis (XP_001937502.1) |
653/417 |
V1908 (f): CACAGTGGAGCAAGGCATCC |
HQ834399–HQ834414/HQ834415 |
| V1909 (r):
ACATACCTAGGCGTCAAGTGC | ||||
|
SRP72
|
A.
dermatitidis (EEQ90678.1) |
941/640 |
V1927 (f): AAGGGAAGGTTGGAGAGACTC |
HQ834416–HQ834431/HQ834432 |
| V1895 (r):
CAAGCAGCATTGTACGCCGTC | ||||
| VPS13 | Verticillium albo-atrum (XP_003001174.1) | 665/545 | V1922 (f): GAGACAACGCTTGTTTGCAAGG | HQ834433–HQ834448/HQ834449 |
| V1923 (r): ACATGCGTCGTTCCAAGATCTG |
*Genes: ALR, α-L-rhamnosidase; Bpntase, 3′(2′),5′-bisphosphate nucleotidase; DHC1, Dynein heavy chain; GPHN, Gephyrin, molybdenum cofactor biosynthesis protein; PCS, peroxisomal-coenzyme A synthetase; POB3, FACT complex subunit; SRP72, signal recognition particle protein 72; VPS13, vacuolar protein sorting-associated protein. †f, forward; r, reverse.