Fig. 4.
Capsaicin-induced DNA condensation and damage in FaDu cells. (A) DAPI staining indicating apoptotic nuclei. FaDu cells treated with capsaicin for 24 h were observed by fluorescence microscopy using a 488 nm filter. (B) Nuclear DNA fragmentation assessed on a 1.8% agarose gel. FaDu cells (1.2×106/well) were incubated with capsaicin for 24 h or untreated. Lane 1, untreated control cells; lane 2, FaDu cells treated with 100 µM capsaicin; lane 3, FaDu cells treated with 300 µM capsaicin. DAPI, 4, 6-Diamidino-2-phenylindole.