Fig. 2.

Fluoxetine down regulated the expression of TRPC1 protein in ET-1 induced human PASMCs. Western blot results are displayed for TRPC1 (92 kDa) and GAPDH (34 kDa) in the human PASMCs cultured with low-serum DMEM (2% FBS, Blank), ET-1, and fluoxetine with (ET-1+F) and without ET-1 (F) for 72 hours. Data, normalized to the amount of actin, are expressed as mean±SEM (n=7). ^*p<0.01 vs. Blank, ^†p<0.05 vs. ET-1. PASMCs, pulmonary arterial smooth muscle cells; FBS, fetal bovine serum; TRPC, transient receptor potential channels; ET-1, endothelin-1; GAPDH, glyceraldehydes phosphate dehydrogenase; SEM, standard error of mean.