Fig. 3.

Fluoxetine rescued the expression of Kv1.5 mRNA that was down regulated by ET-1 in human PASMCs. PCR amplified products are displayed for Kv1.5 (306 bp) and GAPDH (232 bp) in the human PASMCs cultured with low-serum DMEM (2% FBS, Blank), ET-1, and fluoxetine with (ET-1+F) and without ET-1(F) for 72 hours. Data, normalized to the amount of GAPDH, are expressed as mean±SEM (n=7). ^*p<0.05 vs. Blank, ^†p<0.001 vs. ET-1, ^‡p<0.001 vs. Blank. PASMCs, pulmonary arterial smooth muscle cells; ET-1, endothelin-1; GAPDH, glyceraldehydes phosphate dehydrogenase; DMEM, dulbecco's modified eagle medium; SEM, standard error of mean; PCR, polymerase chain reaction.