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. Author manuscript; available in PMC: 2012 Oct 4.
Published in final edited form as: Biochemistry. 2011 Sep 9;50(39):8264–8269. doi: 10.1021/bi201284u

Fig. 1. Global fit to the data of Michaelis and Menten.

Fig. 1

The data from Michaelis and Menten (reproduced in Table 1) were fit by simulation using KinTek Explorer software (9) with the only variable being kcat*E0 to get the smooth lines; an arbitrary, low enzyme concentration was chosen to perform the simulation. Data are for starting concentrations of sucrose of 20.8 (▴), 41.6 (▾), 83 (◆), 167 (■), and 333 (●) mM from Table 1. Data at times longer than 250 m were included in the fit but are not displayed in the figure. The dashed lines show the kinetics predicted if product inhibition is ignored.