Table 1.
Data Collection and Refinement Statistics
| Complex 1 | Complex 2 | Complex 3 | Complex 4 | |
|---|---|---|---|---|
| CUGBP1 RRM | RRM1/2 | RRM1/2 | RRM1/2 | RRM1 |
| RNA sequence | GUUGUUUUGUUU | GUUGUUUUUGUU | UGUGUGUUGUGUG | GUUGUUUUGUUU |
| Data collection | MAD | MAD | Native | Native |
| Space group | I222 | I222 | I222 | P212121 |
| Cell dimensions | ||||
| a, b, c (Å) | 47.6, 70.0, 132.2 | 47.4, 70.1, 131.7 | 47.7, 70.0, 132.6 | 59.2, 62.1, 122.1 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
| Resolution (Å) | 50-1.85 | 50-1.9 | 40-2.2 | 40-2.75 |
| Rsym or Rmerge | 3.6 (36.9)a | 4.2 (36.6) | 12.2 (42.9) | 11.4 (37.6) |
| I/σI | 36.7 (3.6) | 36.3 (3.7) | 11.5 (3.0) | 13.1 (4.6) |
| Completeness (%) | 99.6 | 99.7 | 98.2 | 99.8 |
| Redundancy | 3.8 | 3.8 | 5.5 | 5.3 |
| Refinement | ||||
| Resolution (Å) | 20-1.85 | 20-1.9 | 20-2.2 | 20-2.75 |
| No. reflections | 18,231 | 16,774 | 10,839 | 11,775 |
| Rwork/Rfree | 20.4/24.5 | 20.7/24.7 | 20.1/27.1 | 18.3/26.3 |
| No. residues | ||||
| Protein | 175 | 175 | 175 | 344 |
| RNA | 5 | 5 | 6 | 20 |
| Water | 100 | 98 | 64 | 29 |
| B-factors | ||||
| Protein | 31.5 | 33.6 | 31.4 | 32.0 |
| RNA | 35.1 | 34.9 | 40.8 | 48.3 |
| Water | 37.3 | 40.0 | 35.9 | 26.7 |
| R.m.s. deviations | ||||
| Bond lengths (Å) | 0.008 | 0.007 | 0.010 | 0.007 |
| Bond angles (°) | 1.3 | 1.3 | 1.5 | 1.0 |
a
Values in parentheses are for highest-resolution shell.