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. Author manuscript; available in PMC: 2012 Jun 25.
Published in final edited form as: Structure. 2010 Jan 13;18(1):73–82. doi: 10.1016/j.str.2009.10.015

Figure 1. NMR Spectra and Assignments of human chl1 Intronic 19-mer Sequence in 50 mM K+, 5 mM Phosphate-H2O Buffer, pH 6.8, at 25 °C.

Figure 1

(A) Sequence of the 19-mer human chl1 intronic DNA. (B) Imino proton NMR spectrum of chl1 sequence. Unambiguous guanosine imino proton assignments are listed over the spectrum. (C) Guanosine imino protons were assigned in 15N-filtered NMR spectra of samples containing 2% uniformly-15N-labeled guanosines at the indicated positions. (D) A schematic indicating long-range J couplings used to correlate imino and H8 protons within the guanosine base. (E) H8 proton assignments of chl1 intronic sequence by through-bond correlations between guanoisne imino and H8 protons via 13C5 at natural abundance, using long-range J couplings shown in (D).