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. Author manuscript; available in PMC: 2012 Jun 25.
Published in final edited form as: Methods Mol Biol. 2012;757:451–468. doi: 10.1007/978-1-61779-166-6_26

Fig. 4.

Fig. 4

PKB activation in response to chemoattractant stimulation. (a) Schematic representation of the activation of PKBR1 and PKBA. The N-ternimus of PKBR1 is myristoylated while the N-terminus of PKBA has a PIP3-specific PH domain. Upon cAMP stimulation, the two PKBs are activated through phosphorylation of their HMs by TorC2 and ALs by PDK. The phosphorylation sites (P) can be detected by phospho-specific antibodies. (b) Wildtype cells were stimulated with cAMP, lysed at the indicated time points, and subjected immunoblotting using antibodies against phospho HM (top panel), phospho-AL (middle panel), and phospho-substrate (bottom panel).