Interclass variations of intra-retinal layers manually segmented by three graders. Eight eyes of 4 subjects were imaged in three independent sessions. Three graders manually segmented all 9 intra-retinal layers. No significances were found among graders. The results correlated well. The nine layers of the retina are: the retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), the photoreceptor inner segment (IS), the photoreceptor outer segment (OS), and the retinal pigment epithelium (RPE). ; ; . confidence interval.