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Dissociation curve analysis of the diagnostic nested real-time reverse transcription PCR of West Nile virus isolated from a mare and fetus with fatal neurologic disease, South Africa, 2010. Positive control (Kunjin L1b); SAE74/10 (fetus); SAE75/10 (mare). Expected melting peak of lineage 2 = lineage 1b+6°C; lineage 1a = lineage 1b+10°C.
