Figure 1.

Model system for gene editing in mammalian cells. The target enhanced green fluorescent protein (eGFP) sequence contains a TAG stop codon in place of the wild-type TAC tyrosine codon (bold and underlined). The mutant eGFP that is produced is truncated and nonfluorescent. Specific DNA oligonucleotides (ODNs) were designed that can hybridize to the nontranscribed (NT) strand (72NT, 72NT-U, 72NT-PM). 72NT-PM is a perfect-match ODN that is designed to bind the target NT strand but not elicit a base-exchange event. 72NS is a random sequence ODN bearing no complementarity to the eGFP gene. *Phosphorothioate linkages and the bold capitalized letter indicates the target base.