Figure 2.

Gene editing in HCT116-19 cells. (a) HCT116-19 cells were synchronized with aphidicolin for 24 hours and then washed prior to the introduction of the indicated amount of DNA oligonucleotides (ODN); 72NT or 72NS. Gene editing activity was measured by the emergence of enhanced green fluorescent protein (eGFP) by flow cytometry analysis, 24 hours after the addition of the ODN. Correction efficiency (%) was determined as the percent of eGFP positive cells in the overall viable population. (b) HCT116-19 cells were synchronized for 24 hours and electroporated using standard conditions with 8 µmol/l 72NT. Time points were taken at 72, 96, 120, 44, and 168 hours respectively for analysis by FAC of eGFP (corrected cell) expression. Correction percentage was determined as the number of eGFP positive cells divided by the number of live cells in the population.