Fig. 8.

Electrospun scaffolds created with PEO fibers support infiltration of endogenous cells from calvarial organ cultures. After removal of PEO fibers from TRI/PEO scaffolds by washing, scaffolds were placed directly on top of excised calvaria from neonatal mice. After 8 days in culture, the scaffold/calvaria constructs were fixed and vertical sections were stained with DAPI to show cellular nuclei location (it should be noted that the apparent gap between the scaffold and the calvaria is an artifact introducing during processing and sectioning of the samples). A) Endogenous cells were observed within the TRI/PEO scaffolds, while remaining largely on the surface of TRI scaffolds. B) Cell infiltration was quantified using a custom MatLab script. On average, endogenous bone cells migrated 63.15 μm into TRI/PEO scaffolds, which was significantly greater than infiltration levels observed on TRI scaffolds (20.06 μm). An ** denotes p < .0001