FIG 7.
Intracellular calcium increase and Drp1 phosphorylation at Ser-616 during LAD2 mast cell degranulation. A, Intracellular calciumlevel was measured after SP stimulation. B, LAD2 cell degranulation with or without extracellular calcium, as measured by β-hex release. C, Western blot of Drp1 phosphorylation at Ser-616 after SP (2 µmol/L) stimulation with or without extracellular calcium. D, Drp1 protein density was normalized against β-actin (n = 3; *P < .05). There was no statistical difference in Drp1 phosphorylation at Ser-616 between control and SP stimulation in the absence of calcium.