Skip to main content
. 2011 Dec 19;590(Pt 5):1101–1119. doi: 10.1113/jphysiol.2011.221358

Figure 5. Effect of vigorous PLC stimulation in cells overexpressing M1R on VMI.

Figure 5

A, typical example of currents elicited by CCh (10 μm) in HEK293 cells transfected with TRPC6 (left), C7 (middle) or C3 (right) channels, with (red trace) and without (black trace) muscarinic receptor type-I (M1R). Membrane potentials were held at −50 mV. Comparison of the activation (B) and inactivation (C) kinetics obtained from A (†P < 0.05 **P < 0.001 by t test). D, co-transfection of M1R eliminates VMI of TRPC6 (left) and C3 (right) currents and reduces C7 currents (middle). E, PI(4,5)P2 catalytic schemes for GqPCR (red arrow: M1R co-transfection, blue arrow: no exogenous GqPCR) and DrVSP (black arrow), which lead to production of DAG/IP3/H+ and PI(4)P/Pi, respectively. F, summary of the mean VMI (r) from Fig. 5D. Blue bars represent the transient inhibition of CCh-evoked (1 μm) currents in HEK293 cells expressing only endogenous M1R. The ratio of the expression vectors (TRPC:M1R) is shown below ‘+M1R’, in parentheses.