Table 2.
Summary of the macroscopic current kinetics
| Channel isoform | Agonist (conc. μm) | Condition (PIP2 conc. μm) | Δact10-90% (s) | Δinac90-50% (s) | n |
|---|---|---|---|---|---|
| TRPC3 | CCh (10) | endo | 19.0 ± 6.1 | 55.6 ± 9.3 | 4 |
| CCh (10) | +M1R | 1.6 ± 0.4 | 25.7 ± 3.4 | 8 | |
| CCh (10) | +M1R + PIP2 (100) | 3.7 ± 0.6 | 38.2 ± 3.0 | 4 | |
| CCh (10) | +M1R + PIP2 (250) | 3.5 ± 1.6 | 48.3 ± 6.3 | 7 | |
| TRPC6 | CCh (10) | endo | 16.2 ± 3.1 | 28.3 ± 4.9 | 10 |
| CCh (10) | +PIP2 (100) | 28.3 ± 4.4 | 60.2 ± 13.5 | 7 | |
| CCh (1) | +M1R | 4.0 ± 0.4 | 12.9 ± 1.3 | 11 | |
| CCh (10) | +M1R | 2.3 ± 0.6 | 7.1 ± 1.6 | 13 | |
| CCh (1) | +M1R + PIP2 (100) | 4.3 ± 0.8 | 16.4 ± 1.7 | 6 | |
| CCh (1) | +M1R + PIP2 (250) | 4.9 ± 0.8 | 29.2 ± 2.1 | 6 | |
| CCh (1) | +M1R + PIP2 (500) | 6.5 ± 1.5 | 32.2 ± 4.0 | 7 | |
| TRPC7 | CCh (10) | endo | 14.1 ± 3.8 | 34.5 ± 6.3 | 7 |
| CCh (10) | +M1R | 1.0 ± 0.1 | 2.3 ± 0.9 | 12 | |
| CCh (10) | +M1R + PIP2 (100) | 1.4 ± 0.5 | 13.8 ± 2.2 | 8 | |
| CCh (10) | +M1R + PIP2 (250) | 1.2 ± 0.2 | 14.5 ± 4.3 | 6 | |
| CCh (10) | +M1R + PIP2 (500) | 1.7 ± 0.4 | 17.0 ± 4.8 | 5 | |
| TRPC6-like (A7r5 cells) | AVP (0.05) | n.t. | 3.4 ± 0.5 | 7.1 ± 2.2 | 8 |
| AVP (0.05) | n.t. + PIP2 (100) | 3.6 ± 0.5 | 13.9 ± 1.5 | 6 | |
| AVP (0.05) | n.t. + PIP2 (500) | 8.8 ± 1.6 | 20.8 ± 5.1 | 7 |
Values represent means ± SEM. n.t., no transfection.