Figure 5.

A, phase contrast images showing the effect of 4 h exposure to 1 mmol l⁻¹ H₂O₂ and/or 1 nmol l⁻¹ IMD to endothelial, smooth muscle and fibroblast cells. Scale bar 100 μm. B, counts of viable cells for the cell-types and treatment conditions in Fig. 5A. n = 4 cell sources (or individual cultures for v-HCFs) run in quadruplicate. *P < 0.01 vs. untreated control and #P < 0.01 vs. H₂O₂ alone for that cell-type. C, indirect immunofluorescence staining of cells exposed to 1 mmol l⁻¹ H₂O₂ (actin, red). With IMD addition a degree of normal cellular structure is maintained, represented by HAECs in the larger micrograph. Scale bar: 10 μm. D, protein carbonyl formation (an indicator of oxidative stress) in HAECs in response to the treatment conditions employed in Fig. 5A. n = 3 cell sources *P < 0.05 vs. control and #P < 0.05 vs. H₂O₂ alone. Representative blot shows, in order, both derivatised and non-derivatised signal for each treatment condition.