Figure 6. Immunofluorescence was employed to examine double-labelling for ASIC₃ and peripherin.

Peripherin was used to label DRG neurons that project thin C-fibres. Representative photomicrographs show ASIC₃ and peripherin staining in DRG neurons of a control rat (top panel) and an occluded rat (bottom panel). Arrows indicate representative cells positive for both ASIC₃ and peripherin after they were merged. The number of double-labelled DRG neurons is greater in occluded rats than in control rats. Scale bar, 50 μm.