Table 1. Cytokine profile of naïve neutrophil and transmigrated neutrophil lysates as determined using CBA analysis.
Levels of inflammatory mediators in the lysates of naïve neutrophils or transmigrated neutrophils were determined using CBA analysis (pg/mg). Data are the mean +/− SEM from a minimum of 3 independent experiments carried out on separate cultures.
Corrected pg/mg | ||
---|---|---|
naïve | TM | |
CD62E | 34±12 | 116±34 |
CD62L | 569±73 | 177±63* |
IL-1α | 13±3 | 12±4 |
TNFα | 24±7 | 19±8 |
MIP-1 | 36±5 | 17±6 |
G-CSF | 22±4 | 23±41 |
MCP-1 | 85±12 | 579±110* |
RANTES | 55±11 | 507±80** |
IL-6 | 7±2 | 46±14* |
IL-1β | 30±6 | 16±5 |
KC | 21±3 | 1159±290* |
IL-10 | 37±27 | 46±10 |
IL-17 | 4±1 | 4±12 |
P<0.05 and
P<0.01 (Student’s unpaired T-test).