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. Author manuscript; available in PMC: 2012 Jun 25.
Published in final edited form as: Oncogene. 2009 Oct 5;29(3):411–420. doi: 10.1038/onc.2009.309

Figure 7.

Figure 7

The expression of hairy/enhancer-of-split related with YRPW motif 1 (HEY1) but not L94M leads to increased sensitivity to cisplatin and doxorubicin. U2OS-HEY1 cells (left panels) or U2OS-L94M cells (right panels) were treated with 1 μg/ml tetracycline (Tet) to induce expression of HEY1 or L94M or with vehicle (Control). After 8 h, the cells were cultured with varying concentrations of cisplatin (a) or doxorubicin (b) for another 72 h in the presence and absence of tetracycline. Subsequently, cell viability was assayed using an 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS)-based assay. Data were plotted relative to the drug-free controls. The results shown represent the averages of results of two independent experiments assayed in quadruplicate±s.d.