Figure 1.
Effect of systemic administration of mito-ABAD peptide on the formation of ABAD-Aβ complex in Tg mAPP mice. A, Brain mitochondria isolated from vehicle-treated non-Tg or Tg mAPP mice, and Tg mAPP mice treated with mito-ABAD-DP or mito-ABAD-RP were subjected to immunoprecipitation with 6E10 antibody followed by immunoblotting with anti-ABAD IgG. Top, Two immunoreactive bands (Mr ∼27 and ∼5 kDa) were detected. Bottom, Immunoblotting for COX IV showing an equal amount of mitochondrial protein used for the experiments. B, C, Quantification of intensity of the immunoreactive bands corresponding to Mr ∼27 (B) and ∼5 kDa (C). n = 3–6 mice per group. *p < 0.01 compared with vehicle- or mito-ABAD-RP-treated mAPP mice.