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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Am J Transplant. 2012 Mar 15;12(7):1708–1719. doi: 10.1111/j.1600-6143.2012.04009.x

Figure 4. Distribution of IgG producing ASCs within spleen, mediastinal lymph nodes and bone marrow of sensitized and non-sensitized recipients.

Figure 4

A. Spleen, BM and mediastinal lymph nodes cells were isolated at indicated time points after transplantation or from naïve B6 mice as a control. The total numbers of IgG-secreting cells per each compartment were determined by ELISPOT assay. N = 4–5 mice per group at each time point. B. Immunohistochemical staining of recipient spleen sections for IgG secreting cells at day 7 post transplant. Spleen tissues were excised, fixed and embedded in paraffin. 5μm sections were prepared and stained with anti-mouse IgG antibodies. Dotted lines indicate the border between white and red pulp. Arrows point to bridging channels with clusters of IgG+ ASCs; “a” = arteriole, “f” = follicle. Original magnification 20x, insets 60x.