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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Am J Transplant. 2012 Mar 15;12(7):1708–1719. doi: 10.1111/j.1600-6143.2012.04009.x

Figure 5. Distribution of antigen-specific IgG producing ASCs in heart allograft recipients is distinct from that in virus-infected mice.

Figure 5

A–B. Heterotopic BALB/c heart transplants were placed into non-sensitized B6 mice or into sensitized B6 recipients four weeks after immunization with BALB/c alloantigens. Spleen, BM and mediastinal lymph nodes cells were isolated at indicated time points after transplantation. The total numbers per compartment of cells secreting Dd-binding IgG were determined by ELISPOT assay (A). The kinetics of Dd-reactive ASC frequencies in the spleen and in the BM of sensitized versus non-sensitized recipients (B). C. B6 mice were intraperitoneally injected with 5 × 106 PFU of the mouse hepatitis virus strain JHMV. The numbers of virus-specific ASCs were determined by ELISPOT assay at indicated time points after infection. N= 4–5 mice per group at each time point.