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C57BL/6 mice were transplanted with either LepR+/+, LepR-/- or DKO (LepR-/-; CCR2-/-) bone marrow at 8 weeks of age. After 4 weeks mice were placed on a 60% diet (HFD) for 6 or 12 weeks (n = 10 per group). GTTs and ITTs were performed after 6 and 12 weeks. Mice were sacrificed after the 6 or 12 week period for analysis of AT macrophage infiltration and inflammation.
