Fig. 3.

Alterations of PreP activity/expression in transgenic AD mice. Cortical mitochondrial PreP extracted from 5-month-old indicated Tg mice degraded the biotin Aβ₄₀ (A), Aβ₄₂ (B), and pF₁β (C), respectively. Densitometry of Aβ_40/42 or pF₁β immunoreactive bands was performed using NIH image program. The upper panels indicate the representative immunoblots for Aβ (A, B) and pF₁β (D). In the presence of oPh, mitochondrial PreP was not able to degrade pF₁β peptide (lane 3 vs. lane 2). E) Immunoblotting of cortical mitochondrial protein from the indicated Tg mice for PreP. Representative immunoblots for PreP and Hsp60 are shown in the upper panel. Hsp60 (mitochondrial marker) was used as protein loading control and mitochondrial rich fractions. n = 4–7 mice per group. All experiments were performed in triplicates.