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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Exp Hematol. 2012 Feb 27;40(7):564–574. doi: 10.1016/j.exphem.2012.02.004

Figure 4. Evaluation of histone acetylation and MK gene expression.

Figure 4

A. Flow cytometric analysis of acetylated histone H3 in primary MK from control cultures and in cultures treated with LBH589 (2.5 nM and 5 nM) for 72 hrs. The shift in the mean AcH3-Alexa 488 fluorescence (x axis) indicates increased H3 acetylation. B. Quantification of relative AcH3 levels in three different experiments ±SD. * p value < 0.05. C and D. mRNAs extracted from undifferentiated CD34+ cells (CD34), from control mature MK (C) and from mature MK treated with 2.5 nM and 5 nM LBH589 during the maturation step (i.e. final 7 days in liquid culture) were reverse transcribed then amplified by quantitative real-time PCR using human GATA-1 (C) and NF-E2 (D) primers. Each column represents the average ±SD of three independent experiments each performed in duplicate and normalized to GAPDH.