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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Immunol. 2012 May 30;189(1):318–327. doi: 10.4049/jimmunol.1103099

Figure 7. Effect of RNA interference of TLR expression on GdBCA induced upregulation of cytokine/growth factor expression in normal differentiated human macrophages.

Figure 7

Differentiated normal human macrophages were transfected with 100 nM siGENOME SMARTpool small interfering RNA (siRNA) specific for TLR4, TLR7 or TLR4+TLR7 with DharmaFECT 4 (3 μl/well) followed by 24 h incubation with 1mM Omniscan® (Omni), gadodiamide (Gado) or ProHance® (ProHan). Expression levels of various chemokines were assessed by real time PCR. Values represent the mean (+/− SD) expression levels of three replicates of three separate experiments with macrophages differentiated from monocytes obtained from three different normal individuals. Note that the data are presented in a semilogarithmic scale. LPS (100 ng/mL) and TNFα (10 μg/mL) were used as positive controls for TLR4 and TNFα activation respectively. C(t) values for cytokines were normalized with β-actin. The saline control levels were arbitrarily set at 100% expression at each time point. Values for other samples are expressed relative to the saline control. Statistical significance of changes in chemokine expression was calculated by comparing each Gd compound to the saline control. *: p<0.05; **: p < 0.01; ***: p<0.001.