Table 1.

Cell cycle dependence of nuclear extensions

	Condition	Percent large budded cells with a single nucleusa	Percent cells with nuclear extensionsb
1	Wild type, untreated	32.17 ± 4.62	21.7 ± 0.56
2	Nocodazole	83.09 ± 1.29	87.5 ± 1.96*
3	Bleomycin	80.81 ± 7.95	55.58 ± 4.77*
4	Hydroxyurea	70.24 ± 3.68	26.75 ± 3.47
5	Wild type at 34°C	8.5 ± 0.71	12.5 ± 3.54
6	cdc16-123 at 34°C	76.5 ± 7.78	84.0 ±1.41*
7	GAL-SWE1c	74.87 ± 7.91	29.38 ± 5.78*
8	Wild type + nocodazolec	73.19 ± 8.58	86.87 ± 4.73
9	Wild type at 34°C	7.0 ± 0	5.0 ± 2.83
10	cdc6-1 at 34°C	73.5 ± 3.54	10.5 ± 6.36
11	rad9Δ	29.94 ± 8.18	26.46 ± 3.47*
12	rad51Δ	43.97 ± 2.67	46.15 ± 9.73
13	rad51Δrad9Δ	27.9 ± 5.17	32.94 ± 7.64*
14	GAL-SPO7+NEM1 in nocodazolec	92.97 ± 3.32	23.37 ± 2.78*
15	Vectors only in nocodazolec,d	93.04 ± 0.33	92.88 ± 3.41
16	GAL-OPI1 in nocodazolec	92.23 ± 2.02	61.91 ± 4.13*
17	Vector only in nocodazolec,d	90.94 ± 4.25	96.85 ± 1.52

^aFor different treatments, a large budded with a single nucleus state reflects a different cell cycle stage. For example, nocodazole treatment and growth of cdc16-123 cells at 34°C arrests cells in mid-mitosis, hydroxyurea and growth of cdc6-1 cells at 34°C arrests cells at early stages of DNA replication, and over expression of SWE1 arrests cells in G2. For untreated cells, large budded cells with a single nucleus are typically in G2 or early mitosis (before anaphase)

^bNumbers are average ± standard deviation, based on at least three biological replicates. Statistical analyses were done using students T-test. In each section, the reference control is shown in bold and the values that are statistically significantly different from the control (p< 0.05) are indicated with an asterisk (*).

^cCells were grown in the presence of galactose.

^dVectors only refers to the same vector backbones used in the construction of the GAL-SPO7 and GAL-NEM1, or GAL-OPI1