Figure 1.

Effect of peptide concentration on refolding recombinant HLA-E. Truncated heavy chain of HLA-E was refolded by the dilution method [14, 30, 31] in the presence of light chain human β₂-microglobulin and varying concentrations of peptides. Gel filtration was used to assess successful refolding by separating monomeric complexes from aggregate species. Refolding levels were calculated as induction ratios (see Materials and Methods). Results presented are representative of three independent experiments.