Light-induced oxidation of BODIPY C11 in a rod outer segment, 60 min after bleaching of rhodopsin. (A) Bright field image of a broken off rod outer segment loaded with BODIPY C11. Bar is 10 μm. (B) and (C), fluorescence images of the outer segment, acquired with 555/617 nm (B) and 490/528 nm (C) excitation/emission filters, respectively. (D) and (E), fluorescence images of the same cell after a total exposure to 16 s of 360 nm light, acquired with 555/617 nm (D) and 490/528 nm (E) excitation/emission filters, respectively. The intensity scaling is the same for images B and D and images C and E.