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. Author manuscript; available in PMC: 2013 Jun 19.
Published in final edited form as: Curr Biol. 2012 May 31;22(12):1057–1065. doi: 10.1016/j.cub.2012.03.066

Figure 7. Enzymatically inactive MEC-17 does not rescue the MT and protofilament defects.

Figure 7

(A) Electron micrographs of ALM neurons in mec-17 and mec-17-atat-2 mutant animals expressing the enzymatically inactive mec-17(dW). Scale bars throughout = 100 nm. Left panel had HPF using 1.5% tannic acid + 0.5% glut; right panel used HPF in osmium/acetone (no tannic acid).

(B) Tannic acid stained mec-17;mec-17(dW), and mec-17;atat-2;mec-17(dW) mutant MTs containing 13-p, 14-p and 15-p protofilaments. HPF using 0.25% tannic acid + 0.5% glut.