Figure 2.
Cleavage of PARP and caspase-3 by PALA: (A) 041 and its derivatives 041-p53 and 041-ddp73; (B) SKOV3 and its derivatives ddp73, #3 and ddp73, #5; (C) PC3 and PC3-p53; (D) A549-shGFP and A549-shp53 and (E) Tu686 cells were treated with 250 µM/L of PALA for the indicated times and total cell lysates were immunoblotted with PARP and caspase-3 antibodies that detect only the cleaved form of PARP and caspase-3. All experiments were repeated three times for reproducibility and representative data are shown.