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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Oncogene. 2012 Mar 19;32(7):920–929. doi: 10.1038/onc.2012.96

Figure 5.

Figure 5

Figure 5

p53 and TAp73 play opposing role in PALA-induced expression of Bim and Noxa: (A) 041 and derivatives; (B) PC3 and PC3-p53 and (C) SKOV3 cells were treated with 250 µM/L of PALA for the indicated times and total cell lysates were immunoblotted with anti-Bim and anti-Noxa. (D) O41 and its derivative cell lines and (E) SKOV3 and its derivatives expressing p53 and dominant negative p73 were treated with 250 µM/L of PALA for 48 or 72 h. Total RNA was used for the expression of Bim mRNA by real time PCR. For (D), p values for #, ##, * and ** are 0.0002, 0.0009, 0.0002 and 0.00002, respectively. For (E), p values for *, ** and *** are 0.0003, 0.0004 and 0.0008, respectively. (F, G) Cells were treated with PALA and expression levels of Noxa mRNA were examined by real time PCR. For (F), p values for * and ** are 0.004 and 0.012, respectively. For (G), p values for *, ** and *** are 0.001, 0.0004 and 0.007, respectively.