Skip to main content
. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Oncogene. 2012 Mar 19;32(7):920–929. doi: 10.1038/onc.2012.96

Figure 6.

Figure 6

PALA-induced apoptosis is mediated via Bim. (A) 041 cells were transduced with either scrambled siRNA (shControl) or shRNA against Bim (shBim) and clones were established by puromycin selection. Total cell lysates from PALA-treated and untreated cells were immunoblotted with anti-Bim. (B) Cells were treated with 250 µM/L of PALA for 6 days and apoptosis was measured by TUNEL. (C) Cells were treated with PALA for 72 h and cleavage of PARP and caspase-3 were examined by Western blotting. (D) Expression of Bim was ablated in SKOV3 cells. (E) SKOV3 cells with ablated Bim were treated with 250 µM/L of PALA for 7 days and apoptosis was measured by TUNEL staining. (F) Cells were treated with PALA for 72 h and cleavage of PARP and caspase-3 were examined by Western blotting. For B and E, average apoptosis from three independent experiments with standard deviation as error bars are shown. p values were determined by Student’s T-test and values <0.05 are considered significant.