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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: Oncogene. 2012 Mar 19;32(7):920–929. doi: 10.1038/onc.2012.96

Figure 8.

Figure 8

Role of inhibition of Bcl-2 in PALA-induced apoptosis: (A) Cells were treated with 250 µM/L of PALA for the indicated times and expression of Bcl-2 was examined by Western blotting. (B) 041 and derivative cell lines were treated with 250 µM/L PALA for 72 h and expression of Bcl-2 mRNA was examined by real time PCR. Experiments were done in triplicate. Average results with standard deviations as error bars are presented. p values were determined by Student’s t-test and values <0.05 were considered significant. * indicates significant and ** not significant. (C) Bcl-2 was overexpressed in 041 cells from a retroviral plasmid and apoptosis was measured after treatment with 250 µM/L of PALA. Average results from three independent experiments are presented. (D) 041 and 041-Bcl2 cells were treated with 250 µM PALA for 72 h and expression of Bcl-2 and cleavage of PARP and caspase-3 were examined by Western blotting.