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. Author manuscript; available in PMC: 2012 Jun 25.
Published in final edited form as: Nat Protoc. 2010 Dec 9;6(1):1–14. doi: 10.1038/nprot.2010.168

Figure 3.

Figure 3

Imaged area and specific spatial reference points. (a) Diagram representing reciprocal positioning of calvarium sutures (vertical line: sagittal suture; undulated line: coronal suture) and bone marrow cavities (gray areas). Note: the drawing is not to scale. The orientation of the head is as shown in Figure 2. (b,c) Examples of SHG signal from two reference points: (b) sagittal suture bifurcation and (c) intersection of sagittal and coronal sutures (right side). Scale bar, 50 µm (×30 objective mounted on our custom-made microscope).