Table 3.

An example series of compensation controls.

Cells and amount	Amount of harvest medium	Antibody
Bone marrow cells from Step 4, 10 µl	190 µl	None, unstained control
Bone marrow cells from Step 4, 10 µl	190 µl	c-Kit, APC (2 µl)
Bone marrow cells from Step 4, 10 µl	190 µl	Sca-1, PB (2 µl)
Bone marrow cells from Step 4, 10 µl	190 µl	CD34, FITC (2 µl)
Bone marrow cells from Step 4, 10 µl	190 µl	Flk2, PE (2 µl)
Lineage-depleted cells from Step 14, 5 µl	245 µl	Streptavidin, PO (0.5 µl)

Unstained and single-color controls are necessary in order to set up appropriate voltage and compensation settings for cell sorting. The same antibodies used for the cell mixture sorting are used, at the same dilutions. Lineage-depleted cells are already labeled by the Lineage cocktail and therefore only require streptavidin staining.