Table 4.
Assessment of accuracy and precision of sunitinib and N-desethyl sunitinib in plasmaa
| Nominal Concentration (ng/mL) | Calculated Concentration (ng/mL) | Accuracy (%) | Bias | Precision (%)
|
RSD (%) Between Run | Recovery (%) | |
|---|---|---|---|---|---|---|---|
| Within Run | Between Run | ||||||
| Sunitinib | |||||||
| 0.1 (LLOQ) | 0.092 ±0.003 | 91.94 | −8.06 | 3.08 | 3.31 | 3.31 | -b |
| 0.3 | 0.306 ± 0.021 | 102.11 | 2.11 | 5.66 | 7.04 | 7.04 | 93.81 |
| 8 | 8.20 ± 0.49 | 102.55 | 2.55 | 2.74 | 6.01 | 6.01 | 97.17 |
| 80 | 80.6 ± 4.7 | 100.78 | 0.78 | 1.88 | 5.86 | 5.86 | 97.39 |
| 80.0 (1:10)c | 81.2 ± 3.9 | 101.50 | 1.5 | 4.16 | 4.79 | 4.79 | -b |
| N-desethyl sunitinib | |||||||
| 0.2 (LLOQ) | 0.191 ± 0.011 | 95.50 | −4.5 | 5.64 | 5.90 | 5.90 | -b |
| 0.6 | 0.609 ± 0.042 | 101.58 | 1.58 | 6.49 | 6.92 | 6.92 | 90.34 |
| 16.0 | 16.6 ± 0.7 | 103.50 | 3.5 | 3.11 | 4.32 | 4.32 | 101.1 |
| 160.0 | 154.0 ± 5.7 | 96.25 | −3.75 | 3.02 | 3.69 | 3.69 | 98.13 |
| 160.0 (1:10)c | 160.8 ± 9.2 | 100.50 | 0.5 | 5.08 | 5.75 | 5.75 | -b |
a
Performed in quintuplicate on three separate days
b
Not determined
c
Sample diluted 1:10 (v/v) prior to analysis