Figure 2.

Inhibition of ROS reverses norepinephrine (NE)-induced PKCε gene repression. H9c2 cells and isolated intact fetal hearts (ex vivo) were treated with 10 μM NE in the absence or presence of NAC (1 mM), apocynin (Apo, 0.5 mM), DPI (10 μM), or rotenone (Rot, 20 μM) for 48 h. Pregnant rats were treated with saline or nicotine via osmotic minipumps from d 4 to 21 of gestation in the absence or presence of NAC (500 mg/kg/d) in the drinking water, and 21 d fetal hearts were isolated for analysis (in vivo). A) H9c2 cells. B) Ex vivo fetal hearts. C) In vivo fetal hearts. Data are means ± se, n = 6. *P < 0.05 vs. control.