Table 1.
Serum levels of 23 cytokines in WT or HO-1−/− mice 5 d after vaccination with either Ad5.H1 or vehicle control (n=4/group)
| Cytokine | WT |
HO-1−/− |
P | ||
|---|---|---|---|---|---|
| Vehicle | Vaccine | Vehicle | Vaccine | ||
| IL1a | 50.4 ± 37.8 | 69.6 ± 31.2 | 22.6 ± 6.4 | 30.8 ± 8.8 | 0.07 |
| IL1b | 382.0 ± 57.0 | 387.4 ± 69.7 | 465.4 ± 218.7 | 2454.3 ± 2585.5 | 0.07 |
| IL2 | 43.2 ± 13.4 | 38.0 ± 43.0 | 10.9 ± 12.9 | 15.8 ± 14.2 | 0.22 |
| IL3 | 6.8 ± 4.4 | 8.3 ± 2.4 | 6.0 ± 5.8 | 133.7 ± 236.7 | 0.19 |
| IL4 | 7.5 ± 3.2 | 6.8 ± 0.8 | 4.7 ± 2.4 | 6.9 ± 2.8 | 0.45 |
| IL5 | 38.3 ± 5.0 | 39.5 ± 12.7 | 33.2 ± 8.1 | 227.5 ± 160.1 | 0.03* |
| IL6 | 8.6 ± 1.4 | 13.4 ± 4.2 | 12.4 ± 13.3 | 235.9 ± 411.0 | 0.047* |
| IL9 | 499.5 ± 84.0 | 540.0 ± 50.3 | 467.7 ± 165.7 | 595.3 ± 36.4 | 0.33 |
| IL10 | 19.2 ± 30.5 | 30.7 ± 61.4 | 39.5 ± 39.4 | 135.3 ± 80.6 | 0.047* |
| IL12 ± p40 | 478.7 ± 149.5 | 396.8 ± 115.8 | 1464.9 ± 951.8 | 4162.1 ± 3948.8 | 0.01* |
| IL12 ± p70 | 327.8 ± 103.2 | 166.1 ± 38.2 | 200.4 ± 100.0 | 371.1 ± 40.6 | 0.01* |
| IL13 | 773.7 ± 228.0 | 887.5 ± 102.7 | 634.1 ± 357.9 | 847.8 ± 147.0 | 0.45 |
| IL17 | 91.9 ± 50.6 | 91.4 ± 21.7 | 49.4 ± 33.1 | 184.0 ± 124.2 | 0.19 |
| Eotaxin | 1932.7 ± 348.3 | 1699.6 ± 602.2 | 1648.1 ± 527.1 | 1949.3 ± 1433.4 | 0.93 |
| G-CSF | 120.5 ± 37.2 | 176.8 ± 122.9 | 92.0 ± 44.0 | 653.7 ± 1056.1 | 0.29 |
| GM-CSF | 172.5 ± 7.1 | 126.1 ± 76.5 | 159.7 ± 31.6 | 122.2 ± 65.1 | 0.68 |
| IFNγ | 130.8 ± 46.3 | 107.9 ± 32.3 | 113.0 ± 22.3 | 177.3 ± 42.6 | 0.23 |
| KC | 50.7 ± 11.0 | 56.1 ± 12.2 | 68.3 ± 10.6 | 77.0 ± 43.4 | 0.26 |
| MCP-1 | 411.8 ± 80.5 | 567.3 ± 115.0 | 608.5 ± 266.6 | 942.1 ± 294.8 | 0.25 |
| MIP1α | 403.1 ± 86.8 | 329.1 ± 36.4 | 420.8 ± 102.6 | 580.1 ± 38.8 | 0.003* |
| MIP1β | 118.3 ± 26.3 | 137.6 ± 15.4 | 153.2 ± 30.7 | 919.7 ± 1227.9 | 0.02* |
| RANTES | 79.9 ± 34.8 | 165.3 ± 100.6 | 56.0 ± 28.4 | 252.0 ± 219.8 | 0.13 |
| TNFα | 348.9 ± 38.4 | 476.4 ± 117.4 | 485.8 ± 152.0 | 5728.7 ± 8652.9 | 0.06 |
Serum levels are given as picograms per milliliter. Values are expressed as means ± sd. P values comparing differences across groups were determined by ANOVA with Kruskal-Wallis post test or nonparametric equivalent.
*
P ≤ 0.05.