Figure 2.

Expression of Notch target genes Hes3 and Hes5 in vivo in Psen1-L166P-knock-in mice. Analysis of the expression of Hes3 (A) and Hes5 (B) genes was performed by multiplex RT-PCR, as described in Material and Methods, on mRNA isolated from the cerebral cortex and hippocampus. Bar graphs depict differential gene expression levels between Psen1-L166P (+/+) and (−/−) mice. Analysis was performed in triplicate and normalized to the Polr2a gene. Group averages are reported as relative mRNA levels (means±sd). No significant expression differences were found (P<0.05) by 2-tailed t test.