Figure 6.

Biochemical characterization of APP YAC × Psen1-L166P mice. A) No significant differences were observed in the processing of PS1 using an anti-PS1-CTF Ab and in the generation of NICD by Western blot analysis. B, C) No significant differences were observed in the levels of full-length AβPP between the different genotypes (B); however, both CTFα (CTF83) and β (CTF99) accumulated at significant levels in the homozygous mice but not in heterozygous mice in the cerebral cortex and hippocampus (B, C). No significant changes were observed in the levels of BACE1 and synaptophysin between the 3 phenotypes.